蚯蚓溶解酶凍干機：蚯蚓纖溶(rong)酶(mei)(mei)(mei)具有直接(jie)水解纖維蛋白(bai)和(he)激**纖維蛋白(bai)溶(rong)酶(mei)(mei)(mei)原(yuan)(yuan)成血纖維蛋白(bai)溶(rong)酶(mei)(mei)(mei)，有抗栓和(he)溶(rong)栓作用。生(sheng)(sheng)(sheng)物凍(dong)(dong)(dong)(dong)(dong)干(gan)(gan)機設備是利(li)用生(sheng)(sheng)(sheng)物真空冷凍(dong)(dong)(dong)(dong)(dong)干(gan)(gan)燥技(ji)術，進行各種生(sheng)(sheng)(sheng)物制(zhi)品凍(dong)(dong)(dong)(dong)(dong)干(gan)(gan)、人參提取凍(dong)(dong)(dong)(dong)(dong)干(gan)(gan)、蛋白(bai)酶(mei)(mei)(mei)凍(dong)(dong)(dong)(dong)(dong)干(gan)(gan)、益生(sheng)(sheng)(sheng)菌凍(dong)(dong)(dong)(dong)(dong)干(gan)(gan)、龜膏凍(dong)(dong)(dong)(dong)(dong)干(gan)(gan)、多肽(tai)凍(dong)(dong)(dong)(dong)(dong)干(gan)(gan)、花青素凍(dong)(dong)(dong)(dong)(dong)干(gan)(gan)、蚯蚓纖溶(rong)酶(mei)(mei)(mei)凍(dong)(dong)(dong)(dong)(dong)干(gan)(gan)等加工生(sheng)(sheng)(sheng)產。其中蚯蚓纖溶(rong)酶(mei)(mei)(mei)凍(dong)(dong)(dong)(dong)(dong)干(gan)(gan)粉(fen)制(zhi)作工藝是經過一系列的前處理后采(cai)用真空冷凍(dong)(dong)(dong)(dong)(dong)干(gan)(gan)燥工藝，得到原(yuan)(yuan)有營養成分和(he)活性物質的蚯蚓纖溶(rong)酶(mei)(mei)(mei)凍(dong)(dong)(dong)(dong)(dong)干(gan)(gan)粉(fen)。

蚯蚓纖溶酶凍干粉制造工藝具(ju)體如下(xia)：

1）制作蚯(qiu)蚓(yin)粗粉：將活蚯(qiu)蚓(yin)饑餓自然吐凈泥沙(sha)，清洗，風(feng)干后低溫粉碎，再進(jin)行過篩(shai)制成蚯(qiu)蚓(yin)粗粉；

  2）制作粗酶(mei)(mei)溶(rong)液(ye)(ye)：將蚯蚓粗粉用磷酸緩沖液(ye)(ye)PBS和(he)氯化鈉溶(rong)液(ye)(ye)均勻后置于2~8℃冷柜靜(jing)置12小時，在經過離心，得(de)到上清(qing)液(ye)(ye)。再將上清(qing)液(ye)(ye)進(jin)行(xing)水浴處理后進(jin)行(xing)**次(ci)離心取上清(qing)液(ye)(ye)，在上清(qing)液(ye)(ye)中加硫(liu)酸銨達(da)靜(jing)置后進(jin)行(xing)第三次(ci)離心，得(de)到蚯蚓纖溶(rong)酶(mei)(mei)粗酶(mei)(mei)溶(rong)液(ye)(ye)；

3）粗酶(mei)(mei)(mei)純(chun)化和修(xiu)(xiu)飾(shi)(shi)(shi)：將蚯(qiu)(qiu)蚓(yin)纖(xian)溶酶(mei)(mei)(mei)粗酶(mei)(mei)(mei)溶液過(guo)Sephadex G75凝膠層析(xi)，經過(guo)沖(chong)液洗脫(tuo)得(de)(de)到蚯(qiu)(qiu)蚓(yin)纖(xian)溶酶(mei)(mei)(mei)純(chun)酶(mei)(mei)(mei)。再加(jia)入活化的PEG修(xiu)(xiu)飾(shi)(shi)(shi)劑和硼酸緩(huan)沖(chong)液，進(jin)行(xing)保溫反應，在經過(guo)磷酸緩(huan)沖(chong)液透析(xi)和離(li)心進(jin)行(xing)上清液純(chun)化，收集活性成分得(de)(de)到蚯(qiu)(qiu)蚓(yin)纖(xian)溶酶(mei)(mei)(mei)修(xiu)(xiu)飾(shi)(shi)(shi)酶(mei)(mei)(mei)；

4）蚯(qiu)蚓(yin)纖(xian)溶(rong)酶冷(leng)凍(dong)(dong)干(gan)燥(zao)：將蚯(qiu)蚓(yin)纖(xian)溶(rong)酶修飾(shi)酶裝盤，料厚(hou)8mm左右，進行(xing)(xing)-45℃低溫(wen)冷(leng)凍(dong)(dong)300min，而后(hou)在(zai)35pa真(zhen)空下，進行(xing)(xing)一次干(gan)燥(zao)控溫(wen)范圍(wei)-30~10℃，升華(hua)干(gan)燥(zao)時間720min,除去大(da)部分(fen)流(liu)離水(shui)，然后(hou)進行(xing)(xing)解析干(gan)燥(zao)控溫(wen)范圍(wei)10~40℃，干(gan)燥(zao)運行(xing)(xing)時間860min。經(jing)中生物凍(dong)(dong)干(gan)機的(de)終點(dian)判斷確定，凍(dong)(dong)干(gan)結束(shu)得到含水(shui)1%以內的(de)蚯(qiu)蚓(yin)纖(xian)溶(rong)酶凍(dong)(dong)干(gan)粉。

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